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A biopsy, which numbs the area before removing a small piece of gum tissue that a laboratory checks for cancer cells

[244] Some long-term effects of nicotine may be irreversible because "it is entirely possible that doses of nicotine achieved in the brains of human smokers can damage or kill mHb [medial habenula] neurons that regulate nicotine avoidance behaviors", but more studies are needed to elucidate this underlying mechanism of nicotine-induced degeneration of the mHb-IPn circuit

This claim is supported by another study by the Health Agency of the UK Department of Health

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Limitations Some participants may have misclassified their cigar type